产地对苦荞活性成分、抗氧化能力和降糖活性的影响

Effect of origin on active ingredients,antioxidant capacity and hypoglycemic activity of Tartary buckwheat

摘要:

为评价产地对苦荞活性成分、抗氧化能力和降糖活性的影响，以7个不同产区苦荞为研究对象，首先测定其活性成分（总多酚、总黄酮和D-手性肌醇）、抗氧化能力（DPPH自由基、ABTS+自由基清除率）和降糖活性（对α-葡萄糖苷酶和α-淀粉酶的抑制率），然后通过主成分分析法和聚类分析法进行多因素统计分析。结果表明，不同产区的苦荞活性成分、抗氧化能力和降糖活性具有显著差异。华北地区的一种苦荞（DT）总多酚〔以没食子酸（GAE）计〕和D-手性肌醇（DCI）含量最高，分别为37.24±0.48 mg (GAE)/g和3.97±0.32 mg(DCI)/g；总多糖含量〔以葡萄糖（DE）计〕最低，为14.42±0.38 mg(DE)/g；对DPPH自由基和ABTS+自由基清除能力最强，半数清除质量浓度为0.630±0.075和0.119±0.008 g/L；对α-葡萄糖苷酶和α-淀粉酶半数抑制质量浓度为1.228±0.056和1.327±0.146 g/L。华中地区的一种苦荞（SQ）总黄酮〔以芦丁（RE）计〕含量最高，为15.47±0.19 mg(RE)/g。西南地区的一种苦荞（QJ）总多糖含量最高，为24.98±0.75 mg(DE)/g。发挥抗氧化能力和降糖活性的活性成分为总多酚、总黄酮和D-手性肌醇。

Abstract:

To evaluate the effect of origin on Tartary buckwheat"s active ingredients, antioxidant capacity, and hypoglycemic activity, Tartary buckwheat in seven different production areas as the object of study, firstly, to determine its active ingredients (total polyphenols, total flavonoids, and D-chiral myo-inositol), antioxidant capacity (DPPH free radicals, ABTS + radical scavenging rate) and hypoglycemic activity (inhibition of α-glucosidase and α-amylase), and then by principal component analysis and cluster analysis for multi-factor statistical analysis. The results showed that the active ingredients, antioxidant capacity, and hypoglycemic activity of Tartary buckwheat from different production areas have significant differences. Tartary buckwheat (DT) in North China, had the highest total polyphenol [in gallic acid (GAE)] and D-chiral inositol (DCI) content, respectively, 37.24±0.48 mg (GAE)/g and 3.97±0.32 mg (DCI)/g; total polysaccharide content [in glucose (DE)] was the lowest, 14.42±0.38 mg (DE)/g; on the DPPH radicals and ABTS + radical scavenging ability is the strongest, half scavenging mass concentration of 0.630±0.075 and 0.119±0.008 g/L; α-glucosidase and α-amylase half of the inhibition of the mass concentration of 1.228±0.056 and 1.327±0.146 g/L. A kind of Tartary buckwheat (SQ) in central China, the total flavonoids [as rutin ( RE)] content was the highest, 15.47±0.19 mg (RE)/g. A buckwheat (QJ) in Southwest China had the highest content of total polysaccharides, 24.98±0.75 mg (DE)/g. The antioxidant capacity and hypoglycemic activity of the active ingredients are total polyphenols, total flavonoids, and D-chiral inositol.