仿生磷脂聚合物及纳米银双功能涂层的构建及防污抗菌性能

Construction of dual-functional coating of imitation mussel of phospholipid polymers and silver nanoparticles and its antifouling and antibacterial properties

摘要:

以多巴胺甲基丙烯酰胺和2-甲基丙烯酰氧乙基磷酸胆碱为单体,通过自由基聚合制备了两性离子共聚物PMPCD,再以多巴胺盐酸盐为锚固剂将其接枝到基材表面,形成防污涂层。随后,聚合多巴胺(PDA)将银离子原位还原成纳米银(AgNPs),实现PDA/PMPCD-AgNPs防污-杀菌双功能涂层的制备。通过SEM和XPS对PDA/PMPCD-AgNPs涂层进行表征,证明了涂层的成功制备。测试结果表明PDA/PMPCD-AgNPs涂层具有优异的亲水性、抗菌性和生物相容性。其中,PDA/PMPCD-AgNPs涂层对大肠杆菌和金黄色葡萄球菌的抗菌率分别高达95%及98%以上,抗蛋白质吸附率达94%,同时能够减少血小板的吸附和活化,并展现出较低的溶血率及优异的细胞活性。因此,该防污抗菌双工能涂层在生物材料领域具有广泛的应用前景。

Abstract:

The zwitteronic copolymer P(MPC-co-DMA) (PMPCD) was synthesized based on the free radical copolymerization using dopamine methacrylamide (DMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) as monomers. The PMPCD was grafted onto substrate surface with dopamine (DA) as the anchor, forming an antifouling coating. Furthermore, silver ions were reduced to silver nanoparticles (AgNPs) by polydopamine (PDA) in situ, achieving the preparation of a dual-functional PDA/PMPCD-AgNPs coating with antifouling and antibacterial properties. The PDA/PMPCD-AgNPs coating was characterized by SEM and XPS, confirming the successful preparation of the coating. According to the results obtained from water contact angle measurements, antibacterial assays and biocompatibility experiments. the introduction of PDA/PMPCD-AgNPs coating significantly enhanced the hydrophilicity, antibacterial efficacy and biocompatibility of the substrate surface. Specifically, the antibacterial rates against Escherichia coli and Staphylococcus aureus were both above 97%, the anti-protein adsorption rate reached 94%. Moreover, it could reduce platelet adsorption and inhibit platelet activation while demonstrating low hemolysis rates and excellent cell viability. Therefore, this dual-functional anti-fouling and antibacterial coating holds wide application prospects in the field of biomaterials.