纳米抗菌敷料的构筑及抗耐药菌性能

Construction and antimicrobial performance of antibacterialnano-dressings against drug-resistant bacteria

摘要:

通过液相分层法制备单层MXene,再加入单层/少层黑磷纳米片(BP),以水热法制备了MXene/BP抗菌复合物(AC)。采用SEM、TEM、XRD、ZETA电位仪对其进行了表征。结果表明AC的成功制备,单层MXene均匀分布在BP表面且两者是通过范德华力结合在一起。然后，将AC与聚乳酸-羟基乙酸(PLGA)共混利用静电纺丝技术制得纳米抗菌敷料(PLGA-AC)。采用SEM、接触角测量仪对PLGA-AC的形貌结构及其亲水性能进行了表征,并评价了PLGA-AC的活性氧(ROS)生成能力、抗菌性能和生物相容性。结果表明，与PLGA （122.9°±10.61°）相比,PLGA-AC （52.05°±0.49°）的水接触角显著降低,表明其优异的亲水性能,在生物相容性能评估中展现出良好的生物相容性和血液相容性，其溶血率仅为0.97%。此外,PLGA-AC在超声作用下优异的ROS生成能力，对耐药细菌的清除能力超过99%。

Abstract:

Monolayer MXene was prepared through liquid-phase layering method, followed by the addition of monolayer/few-layer black phosphorus nanosheets (BP) for the preparation of MXene/BP antibacterial composites (AC) via hydrothermal method, characterized by SEM, TEM, XRD and ZETA potentiometers. The results indicated that successful preparation of AC, meanwhile monolayer MXene uniformly distributed on the surface of BP and both connected together through van der Waals forces. Subsequently, the AC was mixed with poly(lactic-co-glycolic acid) (PLGA) using electrospinning technique to fabricate nano antibacterial film (PLGA-AC). The morphology, structure, and hydrophilicity of PLGA-AC were characterized using SEM and contact angle measuring instruments. Meanwhile the reactive oxygen species (ROS) generation capability, antibacterial performance, and biocompatibility of PLGA-AC have been also evaluated. The results showed that the water contact angle of PLGA-AC (52.05 ° ± 0.49 °) was significantly reduced, indicating its excellent hydrophilicity compared with PLGA (122.9 ° ± 10.61 °). It demonstrated good biocompatibility and blood compatibility in the evaluation of biocompatibility, with a hemolysis rate of only 0.97%. Furthermore, PLGA-AC showcased exceptional ROS generation efficacy under ultrasonic conditions, facilitating the clearance of drug-resistant bacteria by more than 99%.