3种植物精油对UVA诱导的人皮肤成纤维细胞损伤的修复作用

Repair effects of three plant essential oils on UVA-induced human HSF cell damage

摘要:

以永久花精油、古巴香脂精油和依兰依兰精油为研究对象，采用气质联用仪检测三种精油的主要成分，通过CCK8法测定其作用于人皮肤成纤维细胞（HSF）的安全质量浓度，基于UVA诱导的HSF损伤模型，从细胞氧化应激反应、蛋白损伤和炎症反应三方面考察三种精油对损伤细胞的修复作用。结果表明，永久花精油、古巴香脂精油、依兰依兰精油分别检测出54、32、46种成分，主要为烯烃类化合物；三种精油作用于HSF的最高安全质量浓度分别为175、400和300 μg/mL；三种精油均可以降低HSF中丙二醛（MDA）的浓度，在照射前加入低质量浓度（1 μg/mL）依兰依兰精油的作用效果最佳，抑制率达20.15%；三种精油均可以提高HSF中超氧化物歧化酶（SOD）的质量浓度，在照射后加入低质量浓度精油效果最佳，其中质量浓度0.1 μg/mL的古巴香脂精油的促进率高达118.09%；三种精油均可以提高HSF中I型胶原蛋白（COL-I）的质量浓度，在照射前加入低质量浓度精油效果最佳，其中质量浓度1 μg/mL的永久花精油的促进率高达137.10%；三种精油均可以降低HSF中基质金属蛋白酶-1（MMP-1）、前列腺素（PGE2）、白细胞介素-1（IL-1）的质量浓度，在照射后加入低质量浓度精油效果最佳，其中，质量浓度0.1 μg/mL的依兰依兰精油对MMP-1抑制率高达50.67%，质量浓度0.1 μg/mL的古巴香脂精油对PGE2抑制率高达57.60%，质量浓度1 μg/mL的永久花精油对IL-1抑制率高达39.97%。

Abstract:

Taking helichrysum essential oil, Copaifera officinalis oil and Cananga odorata essential oil as the research objects, the chemical constituents of the three essential oils were detected by GC-MS. The safe mass concentration of essential oil on cells was determined by CCK8 method. Based on UVA-induced HSF damage model, the effects of essential oils on UVA-induced cell damage were evaluated from the aspects of cellular oxidative stress response, protein damage and inflammatory response. The results showed that 54, 32 and 46 chemical components were detected in helichrysum essential oil, Copaifera officinalis oil and Cananga odorata essential oil respectively, mainly olefin compounds; the maximum safe mass concentrations were 175 μg/mL, 400 μg/mL and 300 μg/mL, respectively. All the three essential oils reduced the content of MDA in cells, and adding low concentration (1 μg/mL) Cananga odorata essential oil before irradiation had the best effect, with an inhibitory rate of 20.15% for MDA. All the three essential oils increased the SOD content in the cells, and adding low concentration essential oils after irradiation had the best effect, in which the promotion rate of Copaifera officinalis oil with 0.1 μg/mL mass concentration on SOD was as high as 118.09%. All the three essential oils increased the content of COL-I in cells, and adding low concentration essential oils before irradiation had the best effect, in which the promotion rate of helichrysum essential oil with a mass concentration of 1 μg/mL was as high as 137.10%. The three essential oils all reduced the contents of MMP-1, PGE2 and IL-1 in the cells, and the addition of low concentration essential oils after irradiation had the best effect. The performance of the three essential oils was also inconsistent. Among them, the inhibitory rate of Cananga odorata essential oil with 0.1 μg/mL mass concentration on MMP-1 was as high as 50.67%, and the inhibitory rate of Copaifera officinalis oil with 0.1 μg/mL mass concentration on PGE2 was as high as 57.60%. The inhibition rate of helichrysum essential oil with 0.1 μg/mL mass concentration on IL-1 was 39.97%.