HEMA改性PDMS/PES复合膜制备及蛋白吸附性能

Preparation and protein adsorption properties of HEMA modified PDMS/PES composite membranes

摘要:

亲水材料甲基丙烯酸羟乙酯（HEMA）与聚二甲基硅氧烷（PDMS）共聚反应制备得到HEMA-PDMS两亲性聚合材料，将其涂覆于机械性能好的微孔聚醚砜（PES）支撑基膜表面，制得HEMA-PDMS/PES复合膜。利用SEM、FTIR、WCA和TGA对其形貌、表面组成、水接触角和热稳定性进行了表征与测定，结果表明，HEMA-PDMS/PES复合膜表面致密无缺陷，热稳定性好，且膜表面水触角为40°左右，与未改性膜相比减少61.2%，由疏水变为亲水表面。同时，牛血清白蛋白吸附测试结果表明改性后的最大吸附量最多减少了13.494%，且膜表面的吸附等温线符合Langmuir吸附模型，其中，涂层质量分数为4%的复合膜蛋白吸附符合准二级动力学吸附，平衡吸附量为208.678 μg/cm2，与Langmuir吸附模型得到的213.194 μg/cm2相近，此吸附过程涉及复合膜表面与蛋白质之间的电子共用或偏移，吸附过程受到氢键的控制。

Abstract:

HEMA-PDMS/PES composite membrane was synthesized by coating the amphiphilic hydroxyethyl methacrylate (HEMA) and polydimethylsiloxane (PDMS) material, which was obtained from copolymerization of hydrophilic HEMA and hydrophobic PDMS, on the microporous polyethersulfone (PES) support membrane, and characterized via SEM, FTIR, WCA and TGA. For analysis on the morphology, surface composition, water contact angle and thermal stability. The results showed that the HEMA-PDMS/PES composite membranes had a dense and defect-free surface, exhibited good thermal stability, and a water contact angle of 40°, reduced by 61.2% in comparison to the unmodified membrane surface, indicating good surface hydrophilicity. Meanwhile, surface adsorption of bovine serum protein showed that the equilibrium adsorption capacity of HEMA-PDMS/PES surface was reduced by up to 13.494% at most compared with that of unmodified surface, with the adsorption isotherm conforming to the Langmuir adsorption model. The adsorption of BSA by 4H-P/P fits to the quasi-second-order kinetic adsorption, with equilibrium adsorption capacity of 208.678μg/cm2，is similar to that of Langmuir adsorption model (213.194μg/cm2). This adsorption process involves electron sharing or shifting between the surface of the composite membrane and proteins, and the adsorption process is controlled by hydrogen bonding.