基于温敏聚合物荧光纳米温度计的研究进展

Research progress on temperature sensitive polymer fluorescent nano thermometers

摘要:

活细胞中发生大量的生化反应,产生和释放自由能来促进细胞的各种活动。细胞内多余的能量以热能的形式排出体外,导致温度升高,从而诱导基因表达、肿瘤代谢等。用理想的荧光探针在纳米尺度上对细胞内温度进行灵敏测量在生物化学及分析领域是一个巨大的挑战。通过利用聚合物可调临界溶解温度范围和良好的生物相容性的优势,荧光聚合物温度计(FPT)能够在细胞水平上以高时空分辨率准确分析温度,从而引起了人们的广泛关注。本文综述了近年来FPT在细胞中温度测定及成像的应用,总结了FPT的设计和合成策略,深入阐述了适用于细胞内温度传感成像的FPT材料未来发展方向。这不仅为研究温度与细胞器功能之间的内在关系开辟了新的道路,而且为深入了解细胞内的生物过程提供了可能。

Abstract:

A series of biochemical reactions occur in living cells, producing and releasing free energy to drive various cellular activities. Excess energy is released in the form of heat, leading to an increase in temperature and influencing the gene expression, tumor metabolism, etc. The sensitive measurement of cell temperature at the nanoscale using ideal fluorescent probes is a huge challenge in many fields. By utilizing the advantages of adjustable critical dissolution temperature range and good biocompatibility of polymers, fluorescent polymer nanothermometers (FPT) can accurately monitor temperature at the cellular level with high spatial resolution, which has attracted widespread attention. This article summarized the recent applications of FPT in cells, emphasizing the design and synthesis strategies of FPT. A deep understanding of these aspects will help to design unique FPT applied in intracellular temperature sensing and imaging. This not only opens up new avenues for studying the intrinsic relationship between temperature and intracellular organelle function, but also provides possibilities for a deeper understanding of biological processes within cells.