5种中药浸膏体外美白活性及体内应用效果比较

Comparison of the whitening bioactivities of five Chinese herbal extracts in vitro and in vivo

摘要:

为探究甘草、茯苓、红景天、黄芩、白术浸膏的抗氧化及美白活性，寻找具有应用于化妆品潜力的天然活性物质，本文以70%乙醇为溶剂，将五种中草药进行闪氏提取、除杂、浓缩制得相应浸膏；以 1,1-二苯基-2-三硝基苯肼（DPPH）自由基、羟基自由基（·OH）、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐（ABTS+）自由基清除活性和酪氨酸酶活性抑制率为评价指标，考察5种中药浸膏的体外抗氧化及美白活性；通过UV照射豚鼠构建皮肤老化模型，涂抹上述5种中药浸膏后，考察其皮肤亮度后取皮并切片、染色，观察其黑色素量及皮肤组织状态。结果表明，5种中药浸膏中，红景天对·OH、DPPH自由基和ABTS自由基的半抑制质量浓度（IC50）分别为0.4655、0.004 mg/mL和2.077 μg/mL，接近阳性对照品VC；对酪氨酸酶的IC50为0.039 μg/mL，展现出最优的抗氧化活性及酪氨酸酶抑制能力。体内实验结果显示，甘草浸膏给药后皮肤亮度（L）为43.39；切片结果显示，红景天、甘草、黄芩浸膏均有明显的皮肤修复功效，甘草组皮肤内黑色素沉积量最少。通过体内外实验比较，红景天浸膏体外具有很强的应用潜力，而甘草浸膏实际应用效果更好。

Abstract:

In order to explore the antioxidant and whitening activities of licorice, poria cocos, Rhodiola rosea, scutellaria baicalensis and Baizhu extract, and to search for natural active substances with potential to be used in cosmetics, five kinds of Chinese herbs were extracted, purified and concentrated with 70% ethanol as solvent. 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical scavenging activity, hydroxyl radical (·OH) activity,2, 2-diazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) diamiammonium salt (ABTS+) free radical scavenging activity and tyrosinase activity inhibition rate were used as evaluation indexes to investigate the antioxidant and whitening activities of 5 extracts of Chinese herbs in vitro. The guinea pig skin aging model was established by UV irradiation. After applying the above 5 kinds of Chinese medicine extract, the skin brightness was investigated, and then the skin was sliced and stained to observe the amount of melanin and the state of skin tissue. The results showed that the semi-inhibitory mass concentration (IC50) of Rhodiola Rosea against ·OH, DPPH and ABTS free radicals were 0.4655, 0.004 mg/mL and 2.077 μg/mL, respectively, which was close to the positive control product VC. The IC50 of tyrosinase was 0.039 μg/mL, which showed the best antioxidant activity and tyrosinase inhibition ability. The results of in vivo experiment showed that the skin brightness (L) after administration of licorice extract was 43.39; The results showed that the extract of Rhodiola rosea, licorice and scutellaria scutellaria had obvious skin repairing effect, and the amount of melanin deposition in the skin of licorice group was the least. Through the comparison of in vivo and in vitro experiments, Rhodiola extract has strong application potential in vitro, and licorice extract has better practical application effect.