SO3气体辅助提取姜黄精油:组分、动力学、生物活性

SO3 gas assisted extraction of turmeric rhizome essential oil: components, kinetics, and biological activity

摘要:

利用SO3气体预处理协同盐析辅助水蒸馏提取（SSHD）姜黄精油，通过单因素实验和响应曲面法优化预处理和提取条件。采用SEM评估预处理对姜黄微观形态的影响，利用气GC-MS分析精油组分，并评价了精油抗氧化活性。结果表明，SSHD最佳条件为：SO3用量2.0%，预处理温度52 ℃，预处理时间50 min和NaCl浓度为3.3%，此时提取率为8.08 ± 0.23 %，比直接水蒸馏提取（HD）提高41.0%。提取动力学研究表明，SSHD法的最大提取率较HD法提高38.0%，传质速率提高了75.0%，提取时间减半。SEM结果显示预处理后姜黄表面出现很多孔洞。GC-MS分析显示，预处理后Ar-姜黄酮和 β-姜黄酮含量降低，而 α-姜黄烯、姜烯和 β-倍半水芹烯含量增加。SSHD精油对DPPH自由基清除作用明显高于HD精油，其IC50值从7.89 ± 0.72 mg/mL降低到 3.66 ± 0.18 mg/mL，而对ABTS自由基清除作用无明显影响。

Abstract:

SO3 gas pre-treatment and salting out-synergistic assisted hydrodistillation (SSBD) extraction was used to improve extraction efficiency of turmeric rhizome essential oil. The pre-treatment and extraction conditions were optimized by single factor experiments and response surface methodology. The effect of pre-treatment on the micromorphological of turmeric was assessed by SEM, the essential oil components were analyzed with GC-MS, and antioxidant activity of the essential oil was also evaluated. The results showed that the optimal conditions for SSHD were: SO3 dosage 2.0%, pre-treatment temperature 52 ℃, pre-treatment time 50 min, and NaCl concentration 3.3%. Under these conditions, the maximum extraction yield reached 8.08 ± 0.23%, which was 41.0% higher than hydrodistillation extraction (HD). Extraction kinetics studies showed that SSHD increased the maximum extraction yield by 38.0%, enhanced the mass transfer rate by 75.0%, and halved the extraction time. SEM results demonstrated that turmeric rhizome exhibited much pores after pre-treatment. Gas chromatography-mass spectrometry (GC-MS) analysis demonstrated that the contents of ar-turmerone and β-turmerone were decreased, but α-curcumene, zingiberene, and β-sesquiphellandrene were increased after pre-treatment. SSHD significantly enhanced DPPH radical scavenging activity, the IC50 value was decreased to 3.66 ± 0.18 mg/mL from 7.89 ± 0.72 mg/mL, while it had no significant effect on ABTS radical scavenging activity.